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pcdna3 1 ha yy1  (Addgene inc)


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    Addgene inc pcdna3 1 ha yy1
    Pcdna3 1 Ha Yy1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+ha+yy1/pcDNA3%2E1+HA-YY1+(Plasmid+%23104395)/pmc12167490-251-0-8
    Average 93 stars, based on 13 article reviews
    pcdna3 1 ha yy1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: De novo variants in RYBP are associated with a severe neurodevelopmental disorder and congenital anomalies.
    Article Snippet: Monika Weisz-Hubshman, Lindsay C. Burrage, Sharayu V. Jangam, Jill A. Rosenfeld, Sandra von Hardenberg, Anke Bergmann, Manuela Friederike Richter, Malgorzata Rydzanicz, Rafal Ploski, Agnieszka Stembalska, Wendy K. Chung, Rebecca R. Hernan, Foong Y. Lim, Theresa Brunet, Steffen Syrbe, Boris Keren, Solveig Heide, David R. Murdock, Hongzheng Dai, Fan Xia, Shamika Ketkar, Brian Dawson, Vinodh Narayanan, Hillary K. Graves, Undiagnosed Diseases Network, Michael F. Wangler, Carlos Bacino, Brendan Lee

    Article Title: De novo variants in RYBP are associated with a severe neurodevelopmental disorder and congenital anomalies
    Article Snippet: .. For the transfection we used the expression plasmids: PCMV6 Myc-DDK RYBP (Origene), His 6 -Ub expression plasmid (gift from Dr. Dae-Sik Lim 36 ), pcDNA3.1 HA-YY1 (Addgene). .. PCMV6 Myc-DDK RYBP C44W expression plasmid was generated with QuickChange Lightning Site-Directed mutagenesis kit (Agilent) per manufacturer protocol.

    Expressing:

    Article Title: De novo variants in RYBP are associated with a severe neurodevelopmental disorder and congenital anomalies.
    Article Snippet: Monika Weisz-Hubshman, Lindsay C. Burrage, Sharayu V. Jangam, Jill A. Rosenfeld, Sandra von Hardenberg, Anke Bergmann, Manuela Friederike Richter, Malgorzata Rydzanicz, Rafal Ploski, Agnieszka Stembalska, Wendy K. Chung, Rebecca R. Hernan, Foong Y. Lim, Theresa Brunet, Steffen Syrbe, Boris Keren, Solveig Heide, David R. Murdock, Hongzheng Dai, Fan Xia, Shamika Ketkar, Brian Dawson, Vinodh Narayanan, Hillary K. Graves, Undiagnosed Diseases Network, Michael F. Wangler, Carlos Bacino, Brendan Lee

    Article Title: De novo variants in RYBP are associated with a severe neurodevelopmental disorder and congenital anomalies
    Article Snippet: .. For the transfection we used the expression plasmids: PCMV6 Myc-DDK RYBP (Origene), His 6 -Ub expression plasmid (gift from Dr. Dae-Sik Lim 36 ), pcDNA3.1 HA-YY1 (Addgene). .. PCMV6 Myc-DDK RYBP C44W expression plasmid was generated with QuickChange Lightning Site-Directed mutagenesis kit (Agilent) per manufacturer protocol.

    Plasmid Preparation:

    Article Title: De novo variants in RYBP are associated with a severe neurodevelopmental disorder and congenital anomalies.
    Article Snippet: Monika Weisz-Hubshman, Lindsay C. Burrage, Sharayu V. Jangam, Jill A. Rosenfeld, Sandra von Hardenberg, Anke Bergmann, Manuela Friederike Richter, Malgorzata Rydzanicz, Rafal Ploski, Agnieszka Stembalska, Wendy K. Chung, Rebecca R. Hernan, Foong Y. Lim, Theresa Brunet, Steffen Syrbe, Boris Keren, Solveig Heide, David R. Murdock, Hongzheng Dai, Fan Xia, Shamika Ketkar, Brian Dawson, Vinodh Narayanan, Hillary K. Graves, Undiagnosed Diseases Network, Michael F. Wangler, Carlos Bacino, Brendan Lee

    Article Title: Activation of FGFR2 Signaling Suppresses BRCA1 and Drives Triple-Negative Mammary Tumorigenesis That is Sensitive to Immunotherapy.
    Article Snippet: .. The other plasmids used were pcDNA3.1 HA-YY1 (Addgene plasmid No. 104 395), lentiCRISPR v2 (Addgene plasmid No. 52 961), pCMVVSV-G (Addgene plasmid No. 8454), pCMV-dR8.2 dvpr (Addgene plasmid No. 8455), pLKO-shBRCA1 #1 (Addgene plasmid No. 44 594), and pLKOshBRCA1 #2 (Addgene plasmid No. 44 595). .. Transfection and Luciferase Assay: Transfections were performed using Lipofectamine 3000 (L3000015; Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions.

    Article Title: DNA stimulates the deacetylase SIRT6 to mono-ADP-ribosylate proteins with histidine repeats
    Article Snippet: SIRT6 was a gift from Cheryl Arrowsmith (Addgene plasmid #41565; http://n2t.net/addgene:41565;RRID:Addgene_41565 ). pFastBac1 Flag Baf170 was a gift from Robert Kingston (Addgene plasmid #1955; http://n2t.net/addgene:1955 ; RRID:Addgene_1955) ( ). .. PcDNA3.1 HA-YY1 was a gift from Richard Young (Addgene plasmid # 104395; http://n2t.net/addgene:104395 ; RRID:Addgene_104395) ( ). ..

    Article Title: Transcription Elongation Machinery Is a Druggable Dependency and Potentiates Immunotherapy in Glioblastoma Stem Cells.
    Article Snippet: Human YY1expressing plasmid was obtained from VectorBuilder. .. The pcDNA3.1 HA-YY1 was a gift from Richard Young (Addgene plasmid #104395, RRID:Addgene_104395). .. 293FT cells (ATCC Cat# PTA-5077, RRID:CVCL_6911) were used to generate lentiviral particles through co-transfection of the packaging vectors pCMV-dR8.2 (Addgene plasmid #8455, RRID:Addgene_8455) and pCI-VSV-G (Addgene plasmid #1733, RRID:Addgene_1733) using the transfection method based on polyethylenimine (PEI, Polysciences Cat# 23966–1) or LipoD293 In Vitro DNA Transfection Reagent (SignaGen Laboratories Cat# SL100668).

    Article Title: De novo variants in RYBP are associated with a severe neurodevelopmental disorder and congenital anomalies
    Article Snippet: .. For the transfection we used the expression plasmids: PCMV6 Myc-DDK RYBP (Origene), His 6 -Ub expression plasmid (gift from Dr. Dae-Sik Lim 36 ), pcDNA3.1 HA-YY1 (Addgene). .. PCMV6 Myc-DDK RYBP C44W expression plasmid was generated with QuickChange Lightning Site-Directed mutagenesis kit (Agilent) per manufacturer protocol.

    Article Title: DNA stimulates the deacetylase SIRT6 to mono-ADP-ribosylate proteins with histidine repeats.
    Article Snippet: .. SIRT6 was a gift from Cheryl Arrowsmith (Addgene plasmid #41565; http://n2t.net/addgene:41565;RRID:Addgene_41565). pFastBac1 Flag Baf170 was a gift from Robert Kingston (Addgene plasmid #1955; http://n2t.net/addgene:1955 ; RRID:Addgene_1955).74 PcDNA3.1 HA-YY1 was a gift from Richard Young (Addgene plasmid # 104395 ; http://n2t.net/addgene:104395 ; RRID:Addgene_104395).75 The pACeBac1-PARP1 plasmid was generated in a previous study.58 The MeCP2 and NLK genes were synthesized as GeneBlocks by Integrated DNA Technologies (IDT). .. Cloning Jo u n al Pr e-p roo f Q5 High-Fidelity DNA Polymerase (NEB) was used for all PCR amplification steps.

    Article Title: Transcription Elongation Machinery Is a Druggable Dependency and Potentiates Immunotherapy in Glioblastoma Stem Cells
    Article Snippet: Human YY1-expressing plasmid was obtained from VectorBuilder. .. The pcDNA3.1 HA-YY1 was a gift from Richard Young (Whitehead Institute for Biomedical Research; plasmid #104395, RRID:Addgene_104395; Addgene). .. 293FT cells (ATCC catalog no. PTA-5077, RRID:CVCL_6911) were used to generate lentiviral particles through cotransfection of the packaging vectors pCMV-dR8.2 (plasmid #8455, RRID:Addgene_8455; Addgene) and pCI-VSV-G (plasmid #1733, RRID:Addgene_1733; Addgene) using the transfection method based on polyethylenimine (catalog no. 23966-1; Polysciences) or LipoD293 In Vitro DNA Transfection Reagent (catalog no. SL100668; SignaGen Laboratories).

    Generated:

    Article Title: DNA stimulates the deacetylase SIRT6 to mono-ADP-ribosylate proteins with histidine repeats.
    Article Snippet: .. SIRT6 was a gift from Cheryl Arrowsmith (Addgene plasmid #41565; http://n2t.net/addgene:41565;RRID:Addgene_41565). pFastBac1 Flag Baf170 was a gift from Robert Kingston (Addgene plasmid #1955; http://n2t.net/addgene:1955 ; RRID:Addgene_1955).74 PcDNA3.1 HA-YY1 was a gift from Richard Young (Addgene plasmid # 104395 ; http://n2t.net/addgene:104395 ; RRID:Addgene_104395).75 The pACeBac1-PARP1 plasmid was generated in a previous study.58 The MeCP2 and NLK genes were synthesized as GeneBlocks by Integrated DNA Technologies (IDT). .. Cloning Jo u n al Pr e-p roo f Q5 High-Fidelity DNA Polymerase (NEB) was used for all PCR amplification steps.

    Synthesized:

    Article Title: DNA stimulates the deacetylase SIRT6 to mono-ADP-ribosylate proteins with histidine repeats.
    Article Snippet: .. SIRT6 was a gift from Cheryl Arrowsmith (Addgene plasmid #41565; http://n2t.net/addgene:41565;RRID:Addgene_41565). pFastBac1 Flag Baf170 was a gift from Robert Kingston (Addgene plasmid #1955; http://n2t.net/addgene:1955 ; RRID:Addgene_1955).74 PcDNA3.1 HA-YY1 was a gift from Richard Young (Addgene plasmid # 104395 ; http://n2t.net/addgene:104395 ; RRID:Addgene_104395).75 The pACeBac1-PARP1 plasmid was generated in a previous study.58 The MeCP2 and NLK genes were synthesized as GeneBlocks by Integrated DNA Technologies (IDT). .. Cloning Jo u n al Pr e-p roo f Q5 High-Fidelity DNA Polymerase (NEB) was used for all PCR amplification steps.



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    pcdna3 1 ha yy1  (Addgene inc)
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    <t>YY1</t> is essential for CRC cell survival. A and B) HCT-116 cells were tranduced with lentiviral vector expressing YY1-specific Sh-RNA. Cells were lysed and total RNA was collected 48 hours after transduction and YY1-knockdown was analyzed by real-time PCR analysis. C) HCT-116 cells were transduced with lentiviral vector expressing a control non-targetting sh-RNA or YY1-specific Sh-RNA. 4 days after transduction cells were analyzed for survival by flow cytometry upon Annexin-V and 7AAD staining. Numbers in the plot indicate relative percentages of cells.
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    Image Search Results


    YY1 is essential for CRC cell survival. A and B) HCT-116 cells were tranduced with lentiviral vector expressing YY1-specific Sh-RNA. Cells were lysed and total RNA was collected 48 hours after transduction and YY1-knockdown was analyzed by real-time PCR analysis. C) HCT-116 cells were transduced with lentiviral vector expressing a control non-targetting sh-RNA or YY1-specific Sh-RNA. 4 days after transduction cells were analyzed for survival by flow cytometry upon Annexin-V and 7AAD staining. Numbers in the plot indicate relative percentages of cells.

    Journal: bioRxiv

    Article Title: Transcriptional Repression by RelA and Yin Yang1 is essential for survival of Colorectal Cancer Cells

    doi: 10.1101/2025.05.12.653423

    Figure Lengend Snippet: YY1 is essential for CRC cell survival. A and B) HCT-116 cells were tranduced with lentiviral vector expressing YY1-specific Sh-RNA. Cells were lysed and total RNA was collected 48 hours after transduction and YY1-knockdown was analyzed by real-time PCR analysis. C) HCT-116 cells were transduced with lentiviral vector expressing a control non-targetting sh-RNA or YY1-specific Sh-RNA. 4 days after transduction cells were analyzed for survival by flow cytometry upon Annexin-V and 7AAD staining. Numbers in the plot indicate relative percentages of cells.

    Article Snippet: The resulted PCR product (RelA-Hinge) was cloned upstream to 3X-HA YY1 in pcDNA3.1 HA-YY1 plasmid (Addgene #104395) under CMV promoter resulting in the generation of RelA-hinge-3xHA YY1 fusion product.

    Techniques: Plasmid Preparation, Expressing, Transduction, Knockdown, Real-time Polymerase Chain Reaction, Control, Flow Cytometry, Staining

    YY1 represses puma in colorectal cancer cells. A) Total RNA from control and YY1-depleted cells was isolated, reverse transcribed and analyzed for the expression of puma by real time pcr. B) Whole cell lysates from control and YY1-depleted cells were isolated and immnoblotted and puma expression was analyzed as indicated.

    Journal: bioRxiv

    Article Title: Transcriptional Repression by RelA and Yin Yang1 is essential for survival of Colorectal Cancer Cells

    doi: 10.1101/2025.05.12.653423

    Figure Lengend Snippet: YY1 represses puma in colorectal cancer cells. A) Total RNA from control and YY1-depleted cells was isolated, reverse transcribed and analyzed for the expression of puma by real time pcr. B) Whole cell lysates from control and YY1-depleted cells were isolated and immnoblotted and puma expression was analyzed as indicated.

    Article Snippet: The resulted PCR product (RelA-Hinge) was cloned upstream to 3X-HA YY1 in pcDNA3.1 HA-YY1 plasmid (Addgene #104395) under CMV promoter resulting in the generation of RelA-hinge-3xHA YY1 fusion product.

    Techniques: Control, Isolation, Reverse Transcription, Expressing, Real-time Polymerase Chain Reaction

    Both RelA and YY1 repress puma promoter. A) HEK-293T cells were transfected with pgl2-puma luciferase reporter vector alone or in combination with RelA or YY1. Relative luciferase activity was shown as light units observed.

    Journal: bioRxiv

    Article Title: Transcriptional Repression by RelA and Yin Yang1 is essential for survival of Colorectal Cancer Cells

    doi: 10.1101/2025.05.12.653423

    Figure Lengend Snippet: Both RelA and YY1 repress puma promoter. A) HEK-293T cells were transfected with pgl2-puma luciferase reporter vector alone or in combination with RelA or YY1. Relative luciferase activity was shown as light units observed.

    Article Snippet: The resulted PCR product (RelA-Hinge) was cloned upstream to 3X-HA YY1 in pcDNA3.1 HA-YY1 plasmid (Addgene #104395) under CMV promoter resulting in the generation of RelA-hinge-3xHA YY1 fusion product.

    Techniques: Transfection, Luciferase, Plasmid Preparation, Activity Assay

    RelA and YY1 interact to form a complex in colorectal cancer cells. HCT116 cells were lysed and both RelA and YY1 were separately immunoprecipitated and the immunoprecipitated complexes were immunoblotted with indicated proteins. Note the interaction of RelA and YY1.

    Journal: bioRxiv

    Article Title: Transcriptional Repression by RelA and Yin Yang1 is essential for survival of Colorectal Cancer Cells

    doi: 10.1101/2025.05.12.653423

    Figure Lengend Snippet: RelA and YY1 interact to form a complex in colorectal cancer cells. HCT116 cells were lysed and both RelA and YY1 were separately immunoprecipitated and the immunoprecipitated complexes were immunoblotted with indicated proteins. Note the interaction of RelA and YY1.

    Article Snippet: The resulted PCR product (RelA-Hinge) was cloned upstream to 3X-HA YY1 in pcDNA3.1 HA-YY1 plasmid (Addgene #104395) under CMV promoter resulting in the generation of RelA-hinge-3xHA YY1 fusion product.

    Techniques: Immunoprecipitation

    YY1 inhibits RelA-dependent transcription. (A) HEK-293T cells were transfected with a 3X-kb reporter plasmid (NF-kB reporter) either alone or in combination with RelA, RelA + YY1 or with RelA-YY1 fusion construct (B and C). Relative luciferase activity was shown as light units in the form of a bar diagram. Also shown in the figure is a schematic diagram for RelA-hinge-YY1 fusion construct design (C). HEK-293T cells were transfected with the RelA-hinge-YY1 construct (3 different clones) and whole cell lysates prepared were analyzed by immunoblotting for indicated proteins to confirm the expression of RelA-hinge-YY1 fusion protein (B).

    Journal: bioRxiv

    Article Title: Transcriptional Repression by RelA and Yin Yang1 is essential for survival of Colorectal Cancer Cells

    doi: 10.1101/2025.05.12.653423

    Figure Lengend Snippet: YY1 inhibits RelA-dependent transcription. (A) HEK-293T cells were transfected with a 3X-kb reporter plasmid (NF-kB reporter) either alone or in combination with RelA, RelA + YY1 or with RelA-YY1 fusion construct (B and C). Relative luciferase activity was shown as light units in the form of a bar diagram. Also shown in the figure is a schematic diagram for RelA-hinge-YY1 fusion construct design (C). HEK-293T cells were transfected with the RelA-hinge-YY1 construct (3 different clones) and whole cell lysates prepared were analyzed by immunoblotting for indicated proteins to confirm the expression of RelA-hinge-YY1 fusion protein (B).

    Article Snippet: The resulted PCR product (RelA-Hinge) was cloned upstream to 3X-HA YY1 in pcDNA3.1 HA-YY1 plasmid (Addgene #104395) under CMV promoter resulting in the generation of RelA-hinge-3xHA YY1 fusion product.

    Techniques: Transfection, Plasmid Preparation, Construct, Luciferase, Activity Assay, Clone Assay, Western Blot, Expressing

    YY1 is essential for CRC cell survival. A and B) HCT-116 cells were tranduced with lentiviral vector expressing YY1-specific Sh-RNA. Cells were lysed and total RNA was collected 48 hours after transduction and YY1-knockdown was analyzed by real-time PCR analysis. C) HCT-116 cells were transduced with lentiviral vector expressing a control non-targetting sh-RNA or YY1-specific Sh-RNA. 4 days after transduction cells were analyzed for survival by flow cytometry upon Annexin-V and 7AAD staining. Numbers in the plot indicate relative percentages of cells.

    Journal: bioRxiv

    Article Title: Transcriptional Repression by RelA and Yin Yang1 is essential for survival of Colorectal Cancer Cells

    doi: 10.1101/2025.05.12.653423

    Figure Lengend Snippet: YY1 is essential for CRC cell survival. A and B) HCT-116 cells were tranduced with lentiviral vector expressing YY1-specific Sh-RNA. Cells were lysed and total RNA was collected 48 hours after transduction and YY1-knockdown was analyzed by real-time PCR analysis. C) HCT-116 cells were transduced with lentiviral vector expressing a control non-targetting sh-RNA or YY1-specific Sh-RNA. 4 days after transduction cells were analyzed for survival by flow cytometry upon Annexin-V and 7AAD staining. Numbers in the plot indicate relative percentages of cells.

    Article Snippet: The resulted PCR product (RelA-Hinge) was cloned upstream to 3X-HA YY1 in pcDNA3.1 HA-YY1 plasmid (Addgene #104395) under CMV promoter resulting in the generation of RelA-hinge-3xHA YY1 fusion product.

    Techniques: Plasmid Preparation, Expressing, Transduction, Knockdown, Real-time Polymerase Chain Reaction, Control, Flow Cytometry, Staining

    YY1 represses puma in colorectal cancer cells. A) Total RNA from control and YY1-depleted cells was isolated, reverse transcribed and analyzed for the expression of puma by real time pcr. B) Whole cell lysates from control and YY1-depleted cells were isolated and immnoblotted and puma expression was analyzed as indicated.

    Journal: bioRxiv

    Article Title: Transcriptional Repression by RelA and Yin Yang1 is essential for survival of Colorectal Cancer Cells

    doi: 10.1101/2025.05.12.653423

    Figure Lengend Snippet: YY1 represses puma in colorectal cancer cells. A) Total RNA from control and YY1-depleted cells was isolated, reverse transcribed and analyzed for the expression of puma by real time pcr. B) Whole cell lysates from control and YY1-depleted cells were isolated and immnoblotted and puma expression was analyzed as indicated.

    Article Snippet: The resulted PCR product (RelA-Hinge) was cloned upstream to 3X-HA YY1 in pcDNA3.1 HA-YY1 plasmid (Addgene #104395) under CMV promoter resulting in the generation of RelA-hinge-3xHA YY1 fusion product.

    Techniques: Control, Isolation, Reverse Transcription, Expressing, Real-time Polymerase Chain Reaction

    Both RelA and YY1 repress puma promoter. A) HEK-293T cells were transfected with pgl2-puma luciferase reporter vector alone or in combination with RelA or YY1. Relative luciferase activity was shown as light units observed.

    Journal: bioRxiv

    Article Title: Transcriptional Repression by RelA and Yin Yang1 is essential for survival of Colorectal Cancer Cells

    doi: 10.1101/2025.05.12.653423

    Figure Lengend Snippet: Both RelA and YY1 repress puma promoter. A) HEK-293T cells were transfected with pgl2-puma luciferase reporter vector alone or in combination with RelA or YY1. Relative luciferase activity was shown as light units observed.

    Article Snippet: The resulted PCR product (RelA-Hinge) was cloned upstream to 3X-HA YY1 in pcDNA3.1 HA-YY1 plasmid (Addgene #104395) under CMV promoter resulting in the generation of RelA-hinge-3xHA YY1 fusion product.

    Techniques: Transfection, Luciferase, Plasmid Preparation, Activity Assay

    RelA and YY1 interact to form a complex in colorectal cancer cells. HCT116 cells were lysed and both RelA and YY1 were separately immunoprecipitated and the immunoprecipitated complexes were immunoblotted with indicated proteins. Note the interaction of RelA and YY1.

    Journal: bioRxiv

    Article Title: Transcriptional Repression by RelA and Yin Yang1 is essential for survival of Colorectal Cancer Cells

    doi: 10.1101/2025.05.12.653423

    Figure Lengend Snippet: RelA and YY1 interact to form a complex in colorectal cancer cells. HCT116 cells were lysed and both RelA and YY1 were separately immunoprecipitated and the immunoprecipitated complexes were immunoblotted with indicated proteins. Note the interaction of RelA and YY1.

    Article Snippet: The resulted PCR product (RelA-Hinge) was cloned upstream to 3X-HA YY1 in pcDNA3.1 HA-YY1 plasmid (Addgene #104395) under CMV promoter resulting in the generation of RelA-hinge-3xHA YY1 fusion product.

    Techniques: Immunoprecipitation

    YY1 inhibits RelA-dependent transcription. (A) HEK-293T cells were transfected with a 3X-kb reporter plasmid (NF-kB reporter) either alone or in combination with RelA, RelA + YY1 or with RelA-YY1 fusion construct (B and C). Relative luciferase activity was shown as light units in the form of a bar diagram. Also shown in the figure is a schematic diagram for RelA-hinge-YY1 fusion construct design (C). HEK-293T cells were transfected with the RelA-hinge-YY1 construct (3 different clones) and whole cell lysates prepared were analyzed by immunoblotting for indicated proteins to confirm the expression of RelA-hinge-YY1 fusion protein (B).

    Journal: bioRxiv

    Article Title: Transcriptional Repression by RelA and Yin Yang1 is essential for survival of Colorectal Cancer Cells

    doi: 10.1101/2025.05.12.653423

    Figure Lengend Snippet: YY1 inhibits RelA-dependent transcription. (A) HEK-293T cells were transfected with a 3X-kb reporter plasmid (NF-kB reporter) either alone or in combination with RelA, RelA + YY1 or with RelA-YY1 fusion construct (B and C). Relative luciferase activity was shown as light units in the form of a bar diagram. Also shown in the figure is a schematic diagram for RelA-hinge-YY1 fusion construct design (C). HEK-293T cells were transfected with the RelA-hinge-YY1 construct (3 different clones) and whole cell lysates prepared were analyzed by immunoblotting for indicated proteins to confirm the expression of RelA-hinge-YY1 fusion protein (B).

    Article Snippet: The resulted PCR product (RelA-Hinge) was cloned upstream to 3X-HA YY1 in pcDNA3.1 HA-YY1 plasmid (Addgene #104395) under CMV promoter resulting in the generation of RelA-hinge-3xHA YY1 fusion product.

    Techniques: Transfection, Plasmid Preparation, Construct, Luciferase, Activity Assay, Clone Assay, Western Blot, Expressing

    Reagents and tools table

    Journal: EMBO Reports

    Article Title: The L27 domain of MPP7 enhances TAZ-YY1 cooperation to renew muscle stem cells

    doi: 10.1038/s44319-024-00305-4

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: HA-YY1 (human cDNA) , Addgene (Weintraub et al, ) , Catalog #104395.

    Techniques: Recombinant, Modification, Magnetic Beads, Sequencing, Blocking Assay, Plasmid Preparation, Electron Microscopy, Software, Reporter Assay, Imaging, In Situ